Huntingtin 20Q Stable PC12 Cell Line | T6019

abm | Huntingtin 20Q Stable PC12 Cell Line | T6019

Huntington disease (HD) is an autosomally dominant degenerative disorder resulting from polyglutamine expansion (>37units) in the huntingtin gene. The repeat is often localized in the N-terminal region of huntingtin and the resulting N-terminal fragments accumulate in the nucleus and become toxic to the cells.

Biosafety:

II

Organism:

Rat

Source Organ:

Adrenal gland

Growth Properties:

Adherent

Morphology:

Small, Irregular

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Co-transfection of a pCIS expression vector expressing a partial huntingtin cDNA containing 20 CAG repeats and a pCDNA3 expression vector carrying G418 resistance

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C