Immortalized Adult Mouse Dorsolateral Prostate Cells (INK4 -/- DLP) | T0655

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SKU:
T0655
Availability:
5 to 7 Days Shipment
€2,925.00
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Description

abm | Immortalized Adult Mouse Dorsolateral Prostate Cells (INK4 -/- DLP) | T0655

INK4aand p19ARF. Both p16INK4aand p19ARF are specific inhibitors of cyclin-dependent kinases Cdk4 and Cdk6 that regulate cell cycle progression. The cells were isolated from the dorsolateral prostate (DLP), which has simple cuboidal epithelium that is somewhat folded. The luminal secretory cells in the epithelium secrete a homogenous eosinophilic substance into the lumen of the DLP. This cell line is a useful model for studying the prostate stroma-epithelium signalling way, which plays an important role in prostate development and cancer progression.

Immortalization Method:

Isolated from p16INK4aand p19ARF Knockout Transgenic Mice

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Dorsolateral Prostate

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, Gentamycin (50ug/ml) + 1% ITS + Glutamine (1x) + 10-8M DHT, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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