Immortalized Calf Thyrocytes-Ras | T0402
- 5 to 7 Days Shipment
abm | Immortalized Calf Thyrocytes-Ras | T0402
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. This cell line needs to be cultured in collagen coated flasks. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify Ras gene expression in immortalized cell line.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml