Immortalized Feline Vena Cava Endothelial Cells - SV40 & hTERT | T0517

abm | Immortalized Feline Vena Cava Endothelial Cells - SV40 & hTERT | T0517

Immortalization Method:

Co-transduced with non-replicating lentivirus carrying SV40LT and hTERT

BioSafety Level:

II

Organism:

Feline

Species:

Feline

Source Organ:

Vena cava inferior

Organ Type:

Blood Vessel

Growth Properties:

Adherent

Morphology:

Endothelial

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 0.1 mg/ml gentamycin, 1 mM sodium pyruvate (TM057), 1% non-essential amino acids(TM068), 50 ?g/ml endothelial cell growth supplement (ECGS; Biomedical Technologies Inc.), and 10 U/ml heparin (Sigma; H5515) and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunodetection for hTERT and SV40L T antigen

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C