Immortalized Rat Arachnoid Cells - SV40/hTERT | T0248

abm | Immortalized Rat Arachnoid Cells - SV40/hTERT | T0248

Immortalization Method:

Serial passaging and transduction with recombinant retroviruses carrying SV40 Large T antigen and hTERT gene

BioSafety Level:

II

Organism:

Sprawgue-Dawley Rats (21-23 day old female)

Species:

Rat

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Spindle shaped|Cobble-stone

Passage Number:

N/A

Population Doubling:

approximately 35.3 hr

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

vimentin, cytokeratin and desmoplakin

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Immunofluorescence and Western Blotting were used to confirm the presence of SV40 (82kDa) and hTERT (122kDa) expression. 2. Immortalized Rat Arachnoid Cells exhibit spindle-like appearance at low density before reaching confluency and “cobblestone” appearance after becoming fully confluent, which appeared to be identical to its primary counterparts. 3. Immunofluorescence and Western Blotting were used to confirm the presence of arachnoid cell markers such as vimentin, cytokeratin and desmoplakin.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C