Immortalized Human Brown Pre-adipocytes (PAZ6) | T0729

abm | Immortalized Human Brown Pre-adipocytes (PAZ6) | T0729

Immortalization Method:

SV40 large and small T-antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Adipose Tissue

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Epithelial

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

N/A

Donor Age:

Infant

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available from abm. to a final concentration of 8% , 1% Glutamax and 1% Penicillin/Streptomycin Solution .
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Compared preadipocyte and adipocyte expression after terminal differentiation via semi-quantitative RT-PCR (Figure 1). 2) Visualized morphology of differentiated and undifferentiated cells via phase contrast microscopy (Figure 2). 3) Compared the expression profiles of primary and differentiated t0729 cells (Figure 3).

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C