Immortalized Human Fallopian Tube Secretory Epithelial Cells (FT240) | T0762

abm | Immortalized Human Fallopian Tube Secretory Epithelial Cells (FT240) | T0762

R24C and p53 shRNA.

Immortalization Method:

Retroviral transduction with hTERT, CDK4^R24C, and p53 shRNA

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Fallopian tube

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Epithelial

Passage Number:

N/A

Population Doubling:

90 - 100 hours

Seeding Density:

15,000 - 35,000 cells/cm10²

Markers:

PAX8, CK7

Donor Age:

N/A

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is a 1:1 mix of Prigrow III medium available at abm. to a final concentration of 2% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use FBS for cell culture as it can induce phenotypic changes.

Quality Control:

1) Western blot; 2) Immunofluorescent staining; 3) real-time qPCR; 4) Proliferation assay; 5) Clonogenic assay; 6) Anchorage-independent growth assay; 7) Tumourigenic assay; 8) Immunohistochemistry; 9) Array comparative genomic hybridization (aCGH)

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C