Immortalized Human Foreskin Keratinocytes (HFK-16E6E7) | T0723

abm | Immortalized Human Foreskin Keratinocytes (HFK-16E6E7) | T0723

Immortalization Method:

Immortalized with retrovirus containing the E6 and E7 open reading frames of HPV16

BioSafety Level:

II

Organism:

Human

Species:

Human

Source Organ:

Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

neomycin resistance

Donor Age:

Not disclosed

Donor Gender:

Male

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Prigrow X medium available at abm. TM0723.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Analysis of provirus DNA; 2) Immunoprecipitation

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C