Immortalized Human Oral Keratinocytes | T0630

abm | Immortalized Human Oral Keratinocytes | T0630

Immortalization Method:

N/A

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Human Oral Mucosa

Organ Type:

Oral

Growth Properties:

Adherent

Morphology:

Other

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.The base medium for this cell line is the Keratinocyte Medium Kit available at abm, Cat. No TM040.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C