Immortalized Human Gastric Cells (KMU-CS12) | T0661

abm | Immortalized Human Gastric Cells (KMU-CS12) | T0661

Immortalization Method:

Spontaneous immortalization in subsequent culture with activated hTERT

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Stomach

Organ Type:

Stomach

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

Oct-4, Musashi-1, TFF-1, CK8, CK18, EMA

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Keratinocyte-SFM (Gibco-Invitrogen Corporation) supplemented with N-acetyl-Lcysteine (NAC; 2 mmol/l) and L-ascorbic acid 2-phosphate (Asc 2P; 0.2 mmol/l), and Penicillin/Streptomycin Solution to a final concentration of 1% available at abm.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Immunocytochemical staining to assess gene expression; 2) Cell proliferation and differentiation assay; 3) Spectral karyotype analysis (SKY)

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C