Immortalized Human Intervertebral Disc Cartilage Endplate-derived Stem Cells -SV40 | T0009
- 5 to 7 Days Shipment
abm | Immortalized Human Intervertebral Disc Cartilage Endplate-derived Stem Cells -SV40 | T0009
Infected with a non-replicating SV40 lentivirus
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml