Immortalized Human Neonatal Fibroblasts - hTERT | T0344
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abm | Immortalized Human Neonatal Fibroblasts - hTERT | T0344
Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml