Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) | T0752

abm | Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) | T0752

Immortalization Method:

Immortalized with the vector pRetro-E2 containing SV40 large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Cartilage

Organ Type:

Skeletal

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

ALCAM (CD166)

Donor Age:

Adult

Donor Gender:

Pooled from male and female OA patients

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, HEPES to a final concentration of 100 mM, L-glutamine to a final concentration of 2 mM, ascorbic acid to a final concentration of 0.1 mM, Sodium Pyruvate Solution to a final concentration of 0.1 mM, and L-glucose to a final concentration of 2.7 μM.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) qPCR; 2) Flow cytometry; 3) Differentiation assays; 4) Immunocytochemistry

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C