Immortalized Human Pulmonary Artery Endothelial Cells | T0550
- 5 to 7 Days Shipment
abm | Immortalized Human Pulmonary Artery Endothelial Cells | T0550
Serial passaging and transduction with recombinant lentiviruses carrying SV40 gene
18 - 28 hours
10,000 - 20,000 cells / cm10²
The base medium for this cell line is Prigrow I medium available at abm. to a final concentration of 10%, Gentamicin to a final concentration of 10 μg/mL, and Amphotericin B (Fungizone) to a final concentration of 0.25 μg/mL. This cell line needs to be cultured flasks coated with Gelatin Coating Solution (0.1%) . Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify the transgene expression.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml