Immortalized Human Total Liver Cell Population | T0581

abm | Immortalized Human Total Liver Cell Population | T0581

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent with some suspension cells

Morphology:

Other

Passage Number:

N/A

Population Doubling:

48 - 55 hours

Seeding Density:

30,000-40,000 cells/cm10²; Recommend split ratio is 1:2 to 1:3

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%. This cell line needs to be cultured in collagen coated flasks.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C