Immortalized Mouse Cementoblasts | T0047

abm | Immortalized Mouse Cementoblasts | T0047

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

CD-1 Mouse

Species:

Mouse

Source Organ:

Mandibular First Molars

Organ Type:

Oral

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

Bone sialoprotein (BSP), Osteocalcin (OC), Osteopontin (OPN), Type I collagen

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, Penicillin/Streptomycin Solution .
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Expression of PTH-1 was assessed by Northern blot and gene-expression assay. Markers were analyzed by RT-PCR.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C