abm immortalized cells
Immortalized Mouse Mammary Epithelial Cells (iMMEC) | T0451
- SKU:
- T0451
- Availability:
- 5 to 7 Days Shipment
Description
abm | Immortalized Mouse Mammary Epithelial Cells (iMMEC) | T0451
Immortalization Method: Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD BioSafety Level: II Organism: 6- to 8-week old C57B/6 female mice Species: Mouse Source Organ: Mammary Epithelia Organ Type: Breast Growth Properties: Adherent Morphology: Cuboidal Passage Number: 11 Population Doubling: 40 - 50 hours Seeding Density: 25,000 - 40,000 cells/cm10² Markers: β-catenin, E-cadherin, Ep-CAM, CK8 and CK18; note that these cells lack drug-resistance markers Donor Age: N/A Donor Gender: N/A Donor Ethnicity: N/A Propagation: The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration on 10%, 5 µg/ml insulin , 1 µg/ml hydrocortisone, and 5 ng/ml EGF . Carbon dioxide (CO2): 5%, Temperature: 37.0°C. Quality Control: 1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot and immunofluorescence were used to check gene expression of β-catenin, E-cadherin, Ep-CAM, CK8, CK18 and ERα. Shipping Condition: Dry Ice Storage Condition: liquid nitrogen or -180C