Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) | T0298

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T0298
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€4,173.00
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Description

abm | Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) | T0298


Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) Cells were isolated from the Cdc25afl/fl/Cre+ mice in which the CDC25A gene is flanked with loxP inserted at exons 1-3 with the Krt14 promoter-driven Cre recombinase (Cre+) in which the expression of CDC25A is absent. abm also provides the Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) Cells which may be used as a control cell line. The cells are suitable for studies on the CDC25A gene and its expression in cell cycle progression.

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

C57/BL6 Cdc25afl/fl/Cre+ mouse

Species:

Mouse

Source Organ:

Skin

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Cobble-stone

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow XI medium available at abm. , Penicillin/Streptomycin Solution to a final concentration of 1% and 5 ng/ml recombinant human FGF7 (Z100595).
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunoblot for the presence of CDC25A was conducted.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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