abm immortalized cells
Immortalized Porcine Alveolar Macrophage Cells (iPAM61) | T0755
- SKU:
- T0755
- Availability:
- 5 to 7 Days Shipment
Description
abm | Immortalized Porcine Alveolar Macrophage Cells (iPAM61) | T0755
Immortalization Method:
Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit
BioSafety Level:
II
Organism:
Pig (Porcine)
Species:
Pig (Porcine)
Source Organ:
Lung
Organ Type:
Lung
Growth Properties:
Adherent
Morphology:
Fibroblast-like
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
N/A
Markers:
Puromycin resistance
Donor Age:
10 weeks old
Donor Gender:
Not disclosed
Donor Ethnicity:
Not disclosed
Propagation:
The base medium for this cell line is Prigrow II medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) PCR; 2) Immunohistochemistry; 3) Analysis of peptide binding to SLA class II molecules
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C