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Immortalized Porcine Alveolar Macrophage Cells (iPAM61) | T0755

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SKU:
T0755
Availability:
5 to 7 Days Shipment
€4,173.00
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Description

abm | Immortalized Porcine Alveolar Macrophage Cells (iPAM61) | T0755

Immortalization Method:

Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit

BioSafety Level:

II

Organism:

Pig (Porcine)

Species:

Pig (Porcine)

Source Organ:

Lung

Organ Type:

Lung

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Puromycin resistance

Donor Age:

10 weeks old

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

Use of PriCoatTM T25 Flasks or Applied Cell Extracellular Matrix is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow II medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) PCR; 2) Immunohistochemistry; 3) Analysis of peptide binding to SLA class II molecules

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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