abm immortalized cells
Immortalized Preadipocyte Clonal Cell Line SCF1-15; Type 3 | T0811
- SKU:
- T0811
- Availability:
- 5 to 7 Days Shipment
Description
abm | Immortalized Preadipocyte Clonal Cell Line SCF1-15; Type 3 | T0811
Immortalization Method:
Conditionally immortalized. Cells were derived from the Immortomouse and grown at 33°C with 10 units/ml interferon-gamma.
BioSafety Level:
II
Organism:
Mouse (M. musculus)
Species:
Mouse
Source Organ:
Adipose
Organ Type:
Adipose
Growth Properties:
Adherent
Morphology:
Fibroblast-like
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
N/A
Markers:
Characterization markers (qPCR): Mx1, Casp1, Cxcl12
Donor Age:
N/A
Donor Gender:
Male
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, GlutaMAX (Gibco) to a final concentration of 2mM, recombinant mouse IFNG to a final concentration of 10 U/ml and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) qPCR; 2) Oil Red O staining
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C