Immortalized Preadipocyte clonal cell lines PGF3-11; Type 1 | T0815

abm | Immortalized Preadipocyte clonal cell lines PGF3-11; Type 1 | T0815

Immortalization Method:

Conditionally immortalized. Cells were derived from the Immortomouse and grown at 33°C with 10 units/ml interferon-gamma.

BioSafety Level:

II

Organism:

Mouse (M. musculus)

Species:

Mouse

Source Organ:

Adipose

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Epithelial-like

Passage Number:

N/A

Population Doubling:

24 - 34 hours

Seeding Density:

10,000 - 20,000 cells/cm²

Markers:

Characterization markers (qPCR): Wt1, Lrn4, Upk3b.

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, GlutaMAX (Gibco) to a final concentration of 2mM, recombinant mouse IFNG to a final concentration of 10 U/ml and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) qPCR; 2) Oil Red O staining

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C