Immortalized Rat Embryonic Fibroblasts (18IM) | T0354
- 5 to 7 Days Shipment
abm | Immortalized Rat Embryonic Fibroblasts (18IM) | T0354
Transfection of plasmid encoded with GFP-fused S18–2 (GFP-S18–2) carrying a neomycin resistance gene
20,000 – 100,000 cells/cm10²; Split Ratio: 1:2 or 1:3
SSEA-1, Sox2 and Oct3/4
The base medium for this cell line is Prigrow V medium available at abm (TM015). To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 5%, 0.5mg/ml Geneticin (Antibiotic G418) and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
1) Western blot analysis for presence of S18-2 in cells; 2) Immunostaining for expression of markers ; 3) Reverse Transcription PCR for determination of presence of markers
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml