Immortalized Rat Hepatocytes - SV40 | T0078
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abm | Immortalized Rat Hepatocytes - SV40 | T0078
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Doubling time of 72 hours
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow IX medium available at abm Cat. No. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml