Immortalized Rat Luteal Cells-Conditionally (GG-CL) | T0596

abm | Immortalized Rat Luteal Cells-Conditionally (GG-CL) | T0596

Immortalization Method:

Serial passaging and infection by SV-40 tsA209 Temperature Sensitive Mutant virus

BioSafety Level:

II

Organism:

Day 14 pregnant Sprague-Dawley rat

Species:

Rat

Source Organ:

Corpora lutea

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Flattened

Passage Number:

N/A

Population Doubling:

17 - 27 hours

Seeding Density:

30,000 - 40,000 cells/cm10²

Markers:

IL-6, ER-β, GR, 20α-HSD, JAK2

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/streptomycin to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C. The functional expression of the SV40 antigen is induced by culturing the cells in vitro at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate and revert to differentiated phenotype.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Northern analysis and RT-PCR were used to confirm the expression of IL-6, glucocorticoid receptor (GR), estrogen receptor-β (ER-β) and orphan nuclear receptor nur 77. 2) Cells were subjected to treatments of exogenous prolactin, estradiol, and cAMP to examine effects.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C