Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM) | T0622

abm | Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM) | T0622

The Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM) is suitable for KSHV infection related studies, such as the growth deregulation mechanisms, as it is able to cause tumor formation. The cells have been efficiently transformed and are immortalized by KSHV. It expresses vascular endothelial, lymphatic endothelial, mesenchymal, and hemapoietic precursor markers. There is downregulation of Thy1.1 expression, a tumor suppressor. These cells are recommended for research on cancer formation linked to KSHV.

Immortalization Method:

Serial passaging and infection by Kaposi’s sarcoma-associated herpesvirus (KSHV)

BioSafety Level:

II

Organism:

Harlan Sprague-Dawley Rat Embryo

Species:

Rat

Source Organ:

Kidney

Organ Type:

Renal

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

β-catenin, vWF, VEGFR-1, VEGFR-3, LYVE-1, vimentin, CD34, VCAM-1, ICAM-1, CD31

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunofluorescence assay, Western blot, and immunohistochemistry was preformed to detect cell markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C