In vitro DNA Amplification Kit | E031
- 5 to 7 Days Shipment
abm | In vitro DNA Amplification Kit | E031
The In vitro DNA Amplification Kit now provides end users with a faster and more efficient technique for high yield DNA amplification from minimal starting template. With a simple set up and short handling time, this has now become the method of choice for any application requiring high throughput DNA amplification. The hassle-free protocol eliminates the requirement for mini prep DNA isolation, thus significantly reducing the cost and time required to obtain your amplified product from the reaction. Applications of this technology include (but are not limited to) whole genome amplification, DNA library construction, SNP genotyping and in vitro cloning of lethal DNA.
One unit is defined as the amount of Klenow Fragment (3’→5’ Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Restriction enzyme digestion of double-stranded DNA
BbsI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
65°C for 20 min
An E. coli strain that carries the cloned and modified BbsI gene from Bacillus laterosporus (C. Story). The BbsI enzyme recognizes and cuts the sequence GAAGAC(N2)^.
Store at -20°C.