KCC2 Stable HEK293 Cell Line | T3038
- 5 to 7 Days Shipment
abm | KCC2 Stable HEK293 Cell Line | T3038
Potassium-chloride transporter member 5 (SLC12A5, also known as KCC2) is a neuron-specific K-Cl symporter responsible for maintaining ion homeostasis in the central nervous system. The electrochemical chloride gradient established by KCC2 activity is critical in regulating postsynaptic inhibition, as well as in protecting cells from stimulation-induced excitotoxicity. The KCC2 Stable HEK293 cell line stably over-expresses the neuronal-specific K-Cl co-transporter isoform KCC2. In contrast to KCC1, where its activity is stimulated by osmotic swelling, KCC2 activity is increased when stimulated with NEM (N-ethylmaleimide). This cell line is suitable for central nervous system studies in the potential roles of KCC2 in ion homeostatsis.
54 - 64 hours
50,000 -100,000 cells/cm2, Split ratio: 1:2 or 1:3
For Research Use Only
KCC2 (neuronal K-Cl cotransporter isoform)
The base medium for this cell line is Prigrow IV medium available at abm
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
1) Cell line was screened using the 86Rb influx assay to determine KCC2 expressed at cell surface; 2) Western blot was used to examine protein expression
10⁶ cells/1.0 ml