Lhx5 Stable Neuro2 Cell Line | T6277

abm | Lhx5 Stable Neuro2 Cell Line | T6277

Lhx5 is a transcription factor with restricted Mamillary body (MBO) expression. LHX5 is a member of the LHX family of transcription factors acting as important differentiation determinants, and it is strongly expressed in the caudal hypothalamus from very early stages (E9.5) and plays a specific role in forebrain development. Lhx5 has an essential role in several different developmental pathways regulating MBO specification and differentiation. This cell can help study the mechanisms behind how neuronal identity are integrated with upstream regional specification networks. Neuro2 cell line demostrates virus susceptibility to: Herpes simplex virus, Vesicular stomatitis virus, and Humnn poliovirus 1.

Biosafety:

II

Organism:

Mouse

Source Organ:

Brain

Growth Properties:

Adherent

Morphology:

Neuron-like morphology, round cell bodies with branching neurite

Clones:

N/A

Passage Number:

N/A

Population Doner:

24-34 hours

Seeding Density:

N/A

Markers:

Expresses transcription factor Llhx5, drug resistance to neomycin

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow VII medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium 95%, 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) ChIP-Seq; 2) Luciferase assay; 3) Cell cycle analysis; 4) ß-Galactosidase activity detection

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C