Luc-Akt-PH-YFP Stable MCF-7-B2 Stable Cell Line | T3161

abm | Luc-Akt-PH-YFP Stable MCF-7-B2 Stable Cell Line | T3161

Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line express the pleckstrin homology (PH) domain of Akt fused to Renilla luciferase with a YFP-Mem, allowing the ability to monitor the PIP₃ production in real time through the established BRET biosensor pair. In which the Luc-Akt-PH will be recruited to the plasma membrane due to PIP₃ production which then results in an energy transfer between the luciferase and YFP. The cells are suitable for the screening and study of molecules on the PI3K/Akt pathway for diabetes and cancer-related research. This cell line is the can be used with the Luc-Akt-PH Stable MCF7 Cell Line (available at abm, Cat. T3160) for experimental studies.

Biosafety:

II

Organism:

Human

Source Organ:

Mammary Tissue

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Luc-Akt-PH through transfection of Luc-Akt-PH vector and selected with 1 mg/ml geneticin to derive stable cline which was then transfected with the pYFP-Mem vector and underwent selection with 1 mg/ml geneticin and 200 µg/ml hygromycin B

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Selection preformed with 1 mg/ml geneticin & 200 µg/ml hygromycin B; 2) BRET assay utilized

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C