Luc-Akt-PH-YFP Stable MCF-7-B2 Stable Cell Line | T3161

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SKU:
T3161
Availability:
5 to 7 Days Shipment
€4,173.00
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Description

abm | Luc-Akt-PH-YFP Stable MCF-7-B2 Stable Cell Line | T3161

Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line express the pleckstrin homology (PH) domain of Akt fused to Renilla luciferase with a YFP-Mem, allowing the ability to monitor the PIP3 production in real time through the established BRET biosensor pair. In which the Luc-Akt-PH will be recruited to the plasma membrane due to PIP3 production which then results in an energy transfer between the luciferase and YFP. The cells are suitable for the screening and study of molecules on the PI3K/Akt pathway for diabetes and cancer-related research. This cell line is the can be used with the Luc-Akt-PH Stable MCF7 Cell Line (available at abm, Cat. T3160) for experimental studies.

Biosafety:

II

Organism:

Human

Source Organ:

Mammary Tissue

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Luc-Akt-PH through transfection of Luc-Akt-PH vector and selected with 1 mg/ml geneticin to derive stable cline which was then transfected with the pYFP-Mem vector and underwent selection with 1 mg/ml geneticin and 200 µg/ml hygromycin B

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Selection preformed with 1 mg/ml geneticin & 200 µg/ml hygromycin B; 2) BRET assay utilized

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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Additional Information

Size:
10⁶ cells/1.0 ml
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