MspI | E072

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SKU:
E072
Availability:
5 to 7 Days Shipment
€260.65
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Description

abm | MspI | E072

N/A

Concentration:

20000 U/ml

Form:

N/A

Alternate Name:

N/A

Reaction Buffer:

Buffer 1: 75%, Buffer 2: 100%, Buffer 3: 50%, Universal Buffer: 100%
View the buffer compatibility chart for restriction enzymes.

Reaction:

1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) Incubate at 37°C.

Notes:

Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

DNA isolated using standard miniprep methods may be cut with lower activity.

Application:

  • DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs
  • cDNA second-strand synthesis
  • Generate single-stranded DNA probes using random primers
  • Site-directed DNA mutagenesis using synthetic oligonucleotides
  • Dideoxy DNA sequencing of single- or double-stranded DNA templates
  • 3’→5’ exonuclease activity can blunt a 3’-overhang

    Components:

    N/A

    Inactivation:

    N/A

    Source:

    N/A

    Shipping conditions:

    Ice Packs

    Storage conditions:

    -20°C

    Storag Buffer:

    0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 50 mM KCl, 200 µg/ml BSA, pH 7.4 @ 25°C.

    Discount(%):

    30

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    Additional Information

    Size:
    10000 U (500 µl)
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