NF-kB Luciferase Stable RAW264.7 Cell Line | T3015

abm | NF-kB Luciferase Stable RAW264.7 Cell Line | T3015

Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. NF-kB Luciferase Stable RAW264.7 Cell Line has been stably transfected with a 3kB-Luc SV40 reporter, which contains 3 NF-kB sites from the interferon gene upstream of the luciferase coding region. This cell line is useful in investigation of NF-kB activation in monocyte-macrophage models. This cell line should be maintained in 100ug/ml G418.

Biosafety:

II

Organism:

Mouse

Source Organ:

Ascites

Growth Properties:

Adherent

Morphology:

Macrophage

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 35 hours

Seeding Density:

50,000 - 70,000 cells/cm². Recommended split ratio of 1:5

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

Have been tested for luciferase activity

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C