NOD1 Stable Knockout AGS (41H8) Cell Line | T6188

abm | NOD1 Stable Knockout AGS (41H8) Cell Line | T6188

CRISPR/Cas9 gene editing was used to generate NOD1 KO clones. For this, AGS cells were transformed with vectors expressing “Cas9 nickase” nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Stomach

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Puromycin resistance

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) PCR; 2) Pyrosequencing

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C