NotI | E051
- 5 to 7 Days Shipment
abm | NotI | E051
Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 25%, Universal Buffer: 100%
Click here to view the buffer compatibility chart for restriction enzymes.
1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences.
- Students gain hands-on laboratory experience using the CRISPR gene-editing technique.
- Experiment performed in E. coli
- Simple and robust editing occurs in 4 days
- Uses standard reagents and equipment
10 mM Tris-HCl (pH 7.5), 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton® X-100, and 50% (v/v) Glycerol
10000 U (500 µl)