Nt.BstNBI (Bst Nickase) | E042

abm | Nt.BstNBI (Bst Nickase) | E042

Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its recognition sequence.

Concentration:

10 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.

This product is distributed for laboratory research only. Caution: Not for diagnostic use .

Application:

Components:

N/A

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 200 µg/ml BSA and 50% (v/v) Glycerol.