Nt.BstNBI (Bst Nickase) | E042

(No reviews yet) Write a Review
SKU:
E042
Availability:
5 to 7 Days Shipment
€110.00
Frequently bought together:

Description

abm | Nt.BstNBI (Bst Nickase) | E042

Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its recognition sequence.

Concentration:

10 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.

This product is distributed for laboratory research only. Caution: Not for diagnostic use .

Application:

  • Ligation of dsDNA with cohesive termini
  • cDNA cloning of products from second strand cDNA synthesis experiments
  • Alternative to T4 DNA Ligase when blunt-end ligation is not required
  • For ligation of blunt-end fragments use T4 DNA Ligase (abm Cat. No. G467)

  • Note: This product is not suitable for NGS or second strand cDNA synthesis applications.

    Components:

    N/A

    Inactivation:

    N/A

    Source:

    N/A

    Shipping conditions:

    Dry ice

    Storage conditions:

    Store all components at -20°C.

    Storag Buffer:

    10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 200 µg/ml BSA and 50% (v/v) Glycerol.

    View AllClose

    Additional Information

    Size:
    1000 U (100 μl)
    View AllClose