Nt.BstNBI (Bst Nickase) | E042
- 5 to 7 Days Shipment
abm | Nt.BstNBI (Bst Nickase) | E042
Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its recognition sequence.
Enzyme supplied with 10X Reaction Buffer
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
This product is distributed for laboratory research only. Caution: Not for diagnostic use .
Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Store all components at -20°C.
10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 200 µg/ml BSA and 50% (v/v) Glycerol.
1000 U (100 μl)