p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line | T3181

abm | p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line | T3181

Immortalization using polyoma middle-sized T-antigen (PmT) method

Biosafety:

II

Organism:

Mouse

Source Organ:

Heart

Growth Properties:

Adherent

Morphology:

Elongated

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

PECAM1, eNOS, VE-cadherin, and von Willebrand Factor

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Isolated from genetically modified p47^phox knockout mice

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) FACS cell sorting; 2) Characterization by Dil-Ac-LDL staining and immunostaining

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C