Pax6 Stable U251N Cell Line | T6168

abm | Pax6 Stable U251N Cell Line | T6168

Pax6 Stable U251N Cell Line (2.10 R) was generated by a retroviral transduction, to stably reintroduce the expression of PAX6, of the Pax6 KnockOut U251N Cell Line (2.10 KO cells). Infected cells were then selected by 10 µg/ml blasticidin; however, the cells do not need to be kept continuously in blasticidin for selection or growth. With the addition of Dox, the cell line will express EGFP-PAX6. PAX6 KnockOut U251N Cell Line (2.10 KO), available at abm

Biosafety:

II

Organism:

Human

Source Organ:

Brain

Growth Properties:

Adherent

Morphology:

Elongated neuron-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 hours

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Rescued cells selected under blasticidin pressure.
2) Inducible EGFP-PAX6 was done by 10 ng/ml of doxocyclin (Dox) (cat#D3447, Sigma), and visualization by green fluorescence and anti-PAX6 western blot.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C