PML-SP100 Stable Double Knockout HEp-2 Cell Line | T6136

abm | PML-SP100 Stable Double Knockout HEp-2 Cell Line | T6136

PML−/− cells, generated using CRISPR/Cas9 human epithelial type 2 (HEp-2) cells. Considered to originate from a human laryngeal carcinoma, allow recognition of over 30 different nuclear and cytoplasmic patterns. These cells were used to study the respective roles of PML and SP100 on the course of HSV replication.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Other

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

55 - 65 hours

Seeding Density:

20,000 - 30,000 cells/cm²

Markers:

PML knockout, SP100 knockout

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Immunoblot; 2) Immunofluorescence staining

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C