Recombinant Human BRAF (N-GST tag) | Z500040

abm | Recombinant Human BRAF (N-GST tag) | Z500040

BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals . Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF .

Functions:

The specific activity of BRAF was determined to be 2,300 nmol/min/mg in a coupled assay as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

BRAF (Ex9-18), BRAF1, RAFB1, B-raf, B-raf 1, MGC126806, MGC138284

Gene Symbol:

BRAF

Gene ID:

N/A

Accession:

NM_004333

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

69

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.