Recombinant Human CDK4/CyclinD1 (N-GST tag) | Z500071

abm | Recombinant Human CDK4/CyclinD1 (N-GST tag) | Z500071

CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex . CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 .

Functions:

The specific activity of CDK4/CyclinD1 was determined to be 12 nmol/min/mgas per activity assay protocol using protein substrate Rb (773-928).

Organism:

Human

Source:

Insect cells

Alias:

CDK4: CMM3; PSK-J3; MGC14458 CyclinD1: BCL1, PRAD1, U21B31, D11S287E

Gene Symbol:

CDK4/CyclinD1

Gene ID:

N/A

Accession:

NM_000075, NM_053056

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

57

Molecular Weight 2 (kDA):

61

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.