Recombinant Human GRK7 (N-GST tag) | Z500159

abm | Recombinant Human GRK7 (N-GST tag) | Z500159

GRK7 is a member of the G protein-coupled receptor kinase subfamily and is a Ser/Thr protein kinase. GRK7 is specifically expressed in the retina and has been shown to phosphorylate cone opsins and initiate their deactivation . GRK7 catalyzes rhodopsin phosphorylation in a light-dependent manner. GRK7 co-localizes with GRK1 in human cone outer segments. PKA phosphorylates GRK7 at Ser(23) and Ser(36) in vitro and phosphorylation of GRK7 by PKA reduces the ability of GRK7 to phosphorylate rhodopsin in vitro . Since exposure to light causes a decrease in cAMP levels in rod cells, it is proposed that phosphorylation of GRK7 by PKA occurs in the dark.

Functions:

The specific activity of GRK7 was determined to be 2.5 nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

GPRK7

Gene Symbol:

GPRK7

Gene ID:

N/A

Accession:

NM_139209

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

89

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.