Recombinant Human HB-EGF (Heparin Binding EGF) | Z102159

abm | Recombinant Human HB-EGF (Heparin Binding EGF) | Z102159

Human HBEGF (Heparin Binding EGF like growth factor) is a member of the EGF family of peptide growth factors that signal through the EGF receptor, and stimulates the proliferation of smooth muscle cells, fibroblasts, epithelial cells, and keratinocytes. HBEGF is expressed in many cell types and tissues, including bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, glomerular podocytes, keratinocytes, macrophages and certain tumor cells. Mature HBEGF is a soluble peptide that arises from the proteolytic processing of the 208 amino acid type-I transmembrane form and exhibits the ability to specifically bind heparin and heparin sulfate proteoglycans.

Functions:

The ED50, as determined by a cell proliferation assay (using balb/c 3T3 cells) is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Organism:

Human

Source:

E. coli

Alias:

Heparin Binding EGF-like growth factor, HBEGF, Diphtheria toxin receptor, DTR, HBEGF

Gene Symbol:

HBEGF

Gene ID:

1839

Accession:

Q99075

Formulation:

Lyophilized from a 0.2 μm filtered solution in PBS.

Solubility:

It is recommended to reconstitute HBEGF in sterile PBS at 250 μg/mL.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

10,1

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.