Recombinant Human HGF | Z102775
- 5 to 7 Days Shipment
abm | Recombinant Human HGF | Z102775
Hepatocyte growth factor (HGF) is a multifunctional cytokine that was originally described as a mesenchymal-derived factor that regulates cell growth, cell motility, morphogenesis and angiogenesis through activation of its receptor, the transmembrane tyrosine kinase encoded by the c-Met proto-oncogene. HGF and c-Met are often co-expressed or over-expressed in a variety of human malignancies including glioblastoma and medullablastoma. The multifunctional effects of HGF: c-Met signaling in tumor cells are mediated by a network of signal transduction pathways including mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K). HGF consists of two subunits held by a disulfide bond. The alpha subunit (69 kDa) has a length of 440 amino acids and the beta subunit (34 kDa) has a length of 234 amino acids. The beta chain of the factor shows approximately 38% homology at the protein level to the serine protease domain of plasminogen. Recombinant HGF expressed in human 293 cells and has a molecular mass of 70 kDa, lower compared to the Molecular mass for this cytokine expressed in CHO cells which emphasizes the difference in post translational modifications.
The EC(50) as determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 was found to be ≤ 20 ng/mL.
HGF, Hepatopoietin-A, HPTA, Scatter factor, SFRecombinant Human Hepatocyte Growth Factor (HGF)
Lyophilized from a solution ontaining 10mM Sodium phosphate, 150mM NaCl, 0.01% Tween 80 and 100mM L-Arginine, pH 6.5
A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight (kDa):
Molecular Weight 2 (kDA):
Molecular Weight 3 (kDA):
>95% as determined by SDS-PAGE
<1 EU/µg of recombinant protein as determined by the LAL method
Shipping Condition :
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.