Recombinant Human IL1B | Z100399

abm | Recombinant Human IL1B | Z100399

Monocytes are the main source of secreted IL1; expressing mostly IL1-beta, while human keratinocytes express large amounts of IL1-alpha. Overall, IL1-beta is the predominant form in humans while IL1-alpha is the one in mice. Both isoforms of IL1 bind to the same receptor and therefore show similar, if not identical, biological activities. The IL1-beta precursor though must be processed before it can bind to the receptor. IL1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. A monomer, IL1B stimulates thymocyte proliferation, B-cell maturation and proliferation, and fibroblast growth factor activity.

Functions:

The ED50 as determined by the dose-dependent inhibition of prostate carcinoma cell line is less than 1ng/ml

Organism:

Human

Source:

E. coli

Alias:

Catabolin, Lymphocyte-activating factor, LAF, Endogenous Pyrogen, EP, IL1F2
Recombinant Human Interleukin-1 Beta (IL1B)

Gene Symbol:

IL1B

Gene ID:

3553

Accession:

P01584

Formulation:

Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and 0.2 mM DTT (pH 8.0)

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

17,5

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.