Recombinant Human MLCK (N-GST tag) | Z500226

abm | Recombinant Human MLCK (N-GST tag) | Z500226

MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal . Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division .

Functions:

The specific activity of MLCK was determined to be 200 nmol/min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

MYLK, KRP, MLCK108, MLCK210, MSTP083, FLJ12216, DKFZp686I10125

Gene Symbol:

MLCK

Gene ID:

N/A

Accession:

NM_053025

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

70

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.