Recombinant Human MYLK3 (N-GST tag) | Z500242

abm | Recombinant Human MYLK3 (N-GST tag) | Z500242

MYLK3 (or myosin light chain kinase 3) plays a key role in rate of cross-bridge recruitment in cardiac myocytes. MYLK3 is upregulated in failing human myocardia and expression of MYLK3 correlates with the pulmonary arterial pressure of patients with heart failure . Knockdown of MYLK3 with small interfering RNAs in cultured rat cardiomyocytes decreases Myl2 phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly . Recombinant murine MYLK3 shows specificity for Myl2 (Mlc2v) and the phosphorylation is calcium- and calmodulin-dependent.

Functions:

The specific activity of MYLK3 was determined to be 9 nmol/min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

caMLCK; MLCK, MLCK2, MGC126319, MGC126320

Gene Symbol:

MYLK3

Gene ID:

N/A

Accession:

BC109097

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

135

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.