Recombinant Human PI3K (p110 alpha/p85 alpha) (N-His tag) | Z500289

abm | Recombinant Human PI3K (p110 alpha/p85 alpha) (N-His tag) | Z500289

The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110a catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants . PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110a is essential for vascular development and inactivation of p110a leads to severe defects in angiogenic sprouting and vascular remodeling .

Functions:

The specific activity of PI3K (p110a/p85a) was determined to be 52 nmol/min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

p110α: PIK3CA, MGC142161, MGC142163, p110-alpha p85α: PIK3R1, GRB1, p85-ALPHA

Gene Symbol:

PI3K (p110a/p85a)

Gene ID:

N/A

Accession:

NM_006218, NM_181523

Formulation:

50mM sodium, pHosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

111

Molecular Weight 2 (kDA):

86

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.