Recombinant Human SHH | Z101677
- 5 to 7 Days Shipment
abm | Recombinant Human SHH | Z101677
Highly conserved proteins, members of the Hedgehog family are widely represented throughout the animal kingdom. The three known mammalian Hedgehog proteins; Sonic (SHH), Desert (DHH) and Indian (IHH) hedgehog share a high degree of sequence and structural identity. SHH is specifically known to be expressed in fetal intestine, liver, lung and kidney. Recombinant Human SHH represents the N-product (N-terminal domain with Cys24 substituted by an Ile-Val-Ile sequence) which retains all the known signalling capabilities.
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1 - 0.4 µg/mL.
Recombinant Human Sonic Hedgehog (SHH)
Lyophilized from a 0.2 μm filtered solution in Phosphate buffer and NaCl
A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Molecular Weight (kDa):
Molecular Weight 2 (kDA):
Molecular Weight 3 (kDA):
>95% as determined by SDS-PAGE
< 1.0 EU/μg of recombinant protein as determined by the LAL method.
Shipping Condition :
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.